Malt agar method for the detection of Alternaria radicina on Daucus carota

摘要

This method was originally published in the ISTA Handbook of Seed Health Testing in November 1964 as S.3. No. 5 and was revised by Gambogi (1987). It has been slightly modified following studies conducted using six seedlots in 11 laboratories by the International Seed Health Initiative - Vegetables in 1999 and 2001 (Van Bilsen, 2003). The studies compared blotter and malt agar methods and concluded that the two were equivalent. The major modification is evaluation after 10 d incubation rather than 7d. Note that seeds can be simultaneously tested for the presence of Alternaria dauci using the same method (see method 7-001 b).