Detection of Pseudomonas savastanoi pv. phaseolicola on Phaseolus vulgaris

摘要

ISTA has published two working sheets (Nos. 65 and 66) for Pseudomonas savastanoi pv. phaseolicola (Psp; Van Vuurde and Van den Bovenkamp, 1987; Jansing and Rudolph, 1996). Not only do the extraction methods in these working sheets differ, they also differ from the stationary overnight soaking commonly used in the testing laboratories of the seed industry. In practice, this has proven to be adequate in terms of sensitivity. Therefore, stationary overnight soaking is part of the present version for the detection of Psp and was incorporated in the validation study (Kurowski and Remeeus, 2007).