Multiplex detection of disease marker proteins with Arrayed ImagingReflectometry

摘要

Arrayed Imaging Reflectometry, or "AIR", is a new label-free optical technique for detecting proteins. AIR relies onbinding-induced changes in the response of an antireflective coating on the surface of a silicon chip. Thus far, we havedemonstrated the use of AIR for the detection of pathogenic E. coli, and for multiplex detection of a broad range ofproteins in human serum. Creation of the near-perfect antireflective coating on the surface of silicon requires carefulcontrol over preparation of the chip surface prior to probe molecule immobilization. We present methods for highlyreproducible, solution-phase silanization and glutaraldehyde functionalization of silicon chips carrying a layer of thermaloxide. Following functionalization with antibodies and passivation of remaining reactive groups, these surfaces provideexceptional performance in the AIR assay.