ITS Sequence Comparison of Germplasm Resources Of Auricularia polytricha

摘要

The internal transcribed spacer (ITS) region in rRNA gene of Auricularia polytricha was cloned and sequenced, and the sequences were compared with those of some common species of Auricularia. The results showed that 6 strains among tested strains, except Teda with different origins, had the same length of ITS2. There were base variances in the ITS region of rRNA gene among the tested strains, and with 11 variant loci in ITS region. Compared with other 4 species from the Gene Bank, ITS1 and ITS2 sequences of A. polytricha varied in great extent. According to the analysis of distance matrix, the genetic relationship between strains and the related known species from systematic dendrogram, ITS sequence analysis supports the traditional classification of Auricularia based on morphology.