Qualitative and Quantitative Study of Gukefuyao Preparation Using FT-IR and HPLC

摘要

Objective To establish methods for the quantitative and qualitative identify of Gukefuyao preparation. Methods Characteristic absorption peaks of Gukefuyao preparation powder and its different extracts were identified, compared and analyzed according to the peaks' shape, position and intensity in infrared spectra. The content of imperatorin was determined by HPLC. Results Fourier transform infrared spectroscopy (FT-IR) Fingerprint of 9 main common peaks was established preliminarily. The mobile phase was acetonitrile-water (62:38) with a flow rate at 1.0mL/min. Detection wavelength was 254nm. The linear ranges for imperatorin was 0.065 ～ 1.032μg. The average recovery was 99.3% (n =6) and RSD was 3.3%. In the meantime, the content of imperatorin in Gukefuyao should not less than 0.05mg/g. Conclusion The methods are simple, rapid and accurate, and can be used for the qualitative and quantitative analysis of Gukefuyao preparation.