The cultivation of Methylobactreium rhodesianum H13 for dichloromethane degradation

摘要

Dichloromethane (DCM) is a toxic pollutant with prolonged persistence in air and water. The work focused on the cultivation of Methylobacterium rhodesiaum H13 which could utilize DCM as the sole carbon source. By comparison and analysis, R2A was chosen as the suitable culture medium for large-scale cultivation. Furthermore, the optimized composition of medium was established on the basis of biomass obtained and the DCM degradation activity of M. rhodesiaum H13, which contained yeast extract 1.5 g/L, peptone 1.5 g/L, glucose 1.5 g/L, sodium pyruvate 0.9 g/L, K_2HPO_4 0.9 g/L and MgSO_4·H_2O 0.15 g/L.