Surface Constructions of Nano-Micro Structured CaP Biomaterials and Their Biocompatibility and Bioactivity

摘要

In this study, we concentrated our attention to develop various methods for surface nano-micro construction of calcium phosphate (CaP) and their composite coatings, including octacalcium phosphate (OCP), hydroxyapatite (HAp), micropatterned titanium surface by using electrochemical techniques. The physico-chemical and biological properties of the as prepared nano-micro structured biomaterials were characterized by SEM, XRD, FT-IR, Raman, in vitro and in vivo evaluations respectively. The SEM patterns show a uniform micro-porous morphology consisting of wirelike crystals at nanometer scale. It is suggested that under controlled prepared conditions, the primary CaP nanowires grow and self- assemble to construct an ordered micro-porous nest-like morphology, thus to form a nano-micro two-level structure. The XRD results indicate that the CaP nanowires are orderly arranged with their c-axis preferentially perpendicular to the substrate surface. The Raman and IR spectra affirm that the main component of the coating is well crystallized HAp and OCP under the different preparations respectively. It is demonstrated that the nano- micro structured CaP composite coatings exhibit a preferable biocompatibility, because they are similar to the natural bone in both chemical composition and micro structure on surface.