Yeast vacuole staining using quinacrine and neutral red

摘要

We describe a group of experiments designed to set up a reproducible and reliable method for in vivo vacuole staining in Saccharomyces cerevisiae using neutral red, a vital dye previously used to stain intracellular organelles from plants and animals. In addition, we have compared the usage of neutral red with the use of quinacrine, a fluorescent dye that can accumulate in acidic compartments too. Advantages and disadvantages of both methods in vacuole acidification assays are discussed.