Investigation into Aerosol Staining of Microbes for Bio-aerosol Online Monitoring

摘要

Bio-aerosols can cause human health effects and diseases. Real-time evaluation of bio-aerosols becomes necessary for sources identification, exposure monitoring and air quality management. An aerosol staining process by mixing of aerosol microbes and fluorescence dye mist was investigated to propose a rapid bio-aerosols detection tool. Dry dispersion of a typical microbe aerosol, yeast (Saccharomyces cerevisiae), was mixed directly with fluorescence dye mist, DAPI and Auramine O, in a designed mixing system. The staining process was evaluated by a Spectrofluorometer (SFM) and an UV-OPC (Biotrak?) to detect the change of emission intensity and an autofluorescence signal from the microbe (V/T ratio), respectively. Increases of emission intensity (SFM) and V/T ratio (Biotrak?) for stained yeast refer to that aerosol yeast could be stained by mixing with fluorescence dye mist. The concentration of dye solution was shown to affect to a stained fraction of yeasts.