The effect of solanine on the membrane potential of mitochondria in HepG2 cells and Ca~(2+)i in the cells

摘要

To observe the effect of solanine on the membrane potential of mitochondria in HepG_2 cells and [Ca~(2+)]_i in the cells, and to uncover the mechanism by which solanine induces apoptosis. HepG_2 cells are double stained with TMRE and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca~(2+)]_i in the cells are observed using LCSM.The results of double staining with TMRE and Fluo-3/AM show that solanine can lower membrane potential and increase the concentration of Ca~(2+) in the cells Solanine opens up the PT channels in the membrane by lowering the membrane potential, leading to Ca~(2+) being transported down its concentration gradient, which in turn leads to the rise of the concentration of Ca~(2+) in the cell, turning on the mechanism for apoptosis.