The Study of the Growth of Tissue Cultures under a Layer of Nanotextiles

摘要

Nowadays nanotextiles are very popular due to their unique properties (antibacterial, very light weight, thin, solid, high porosity, transparency, good mechanical properties, etc.). These characteristics bring new possibilities of classification in the various fields of human activity. For example, the filtration of air and water through the nanofiber membranes whose structure is similar to the construction of tissue that supports the growth of cells in their natural environment. Carbon nanofibers have their application in treating the effects of myocardial infarction. Nanotextiles consist of nanofibers with a diameter in the range from 50 to 500 nm. Production of such a fabric is much more complex than classical woven fabric. This material is not possible hold by any device. Production is carried out using the electrospinning. The nanotextile produced spinning method was used on the samples of spruce embryos and concluded (with samples) in the plastic Petri's dishes. Spruces embryos were selected for their rapid growth, their biggest increase is till the fifteenth day then they are growth-stabilized. The aim of this paper is to determine the influence of the nanotextiles to the tissue cultures and whether the embryos will be able to growth under this structure. The samples were periodically removed from the magnetic field and moved in an isolated box throughout the outdoor environment into the room with NMR tomograph for obtaining the images of individual tissue cultures. After fifteen days the last measurement was carried out and the results of all measurements were evaluated using the programs Marevisi and Matlab. Observed parameters of individual samples were processed into graphs.