USE OF LATE EMBRYOGENESIS PROTEINS TO ENGINEER DESICCATION TOLERANCE IN MAMMALIAN CELLS

摘要

The mechanisms by which different anhydrobiotic organisms protect their cellular structure and function during desiccation can be an important strategy for cell stabilization to support cell-based therapies. While it is known that sugars such as trehalose can help in stabilizing biological structures during desiccation (1, 2), recent studies underscore the importance of various protective proteins during drying that include Late Embryogenesis Abundant (LEA) proteins (3, 4). Late embryogenesis abundant (LEA) proteins are associated with desiccation tolerance in several anhydrobiotic organisms including insects, tardigrades and rotifers (3, 4).