Detection and in vitro regulation of NHE1 and NHE3 mRNA expression in rumen epithelia of cows

摘要

NHE (Na+/H+ exchanger) mediates electroneutral Na transport across rumen epithelium by ejection of intracellular H+ in exchange of external Na+ with a ratio of 1:1. It contributes to the absorption of Na+ and maintenance of intracellular pH homeostasis of rumen epithelium. This study explored the existing of NHEs in rumen epithelia of cows and the responses of NHE1 and NHE3 mRNA to IGF-1. The results, obtained by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), illustrated that NHE1 and NHE3 mRNA is present in rumen epithelia of cows. The NHE1 and NHE3 mRNA expression were promoted by IGF-1 treatment (25 ng/ml) in vitro. The abundance of NHE 1 and NHE3 mRNA were significantly higher in IGF-1 treated groups (P＜0.05). Taking together, the current study revealed that the mRNA of NHE1 and NHE3 in rumen epithelia of cows could be positively regulated by IGF-1.