HIGH THROUGHPUT AGROBACTERIUM TRANSFORMATION OF WHEAT: A TOOL FOR FUNCTIONAL GENOMICS

摘要

At Rothamsted Research UK, we have developed a robust protocol for theAgrobacterium-medieLted transformation of elite wheat and during the last year, madeover one hundred independent transgenic lines in one durum and three bread wheatvarieties. We are now able to provide training or generate transgenic wheat lines as anot-for-profit service (see www.bract.org). Approximately 40% of our lines contain asingle, functional T-DNA insertion. This paper describes the current status of Agrobacterium transformation of wheat and some initial molecular analysis of our lines. It alsodescribes our plan to further increase throughput using mature seed-derived callus andother approaches.