Study on different preparation methods of pirarubicin liposomes

摘要

To explore the promising industrialized technique for the preparations of pirarubicin liposomes, pirarubicin was encapsulated into the liposomes using three different preparing techniques, modified transmembrane ammonium sulfate gradient, thin film evaporation and micro-fluidic technique. Therefore the preliminary study of the morphology, particle size distribution, entrapment efficiency and drug loading amount of liposomes were investigated. TEM showed the THP liposomes were small spherical and sphere-like vesicles. Particle size determination showed that the average particle size were about 400(nm), and the micro-fluidic technique of liposomes had mono-disperse size distribution. And the entrapment efficiency of the three techniques were 96.0.%, 79.3% and 86.4% respectively, accordingly the drug loading amount were 4.8%, 4.0% and 4.3%.