Study on cutaneous permeation in vitro of Kushen recipe gel

摘要

Objective: To establish a suitable dosage form for a traditional anti-anaphylaxis Chinese medicine of Kushen recipe. The effect of skin in vitro of the recipe was investigated. Methods: A technique of extracting with ethanol and purifying with absorbent resin to obtain alkaloids from Kushen recipe were adopted, while steam distillation to extract volatile oil. The extraction was made to gel. The skin from SD rats' abdomen was used as permeability barriers. Then the cutaneous permeation effect of the extraction with water, the purifying extraction and the gel were compared by Valia-Chien and Franz diffusion cell method. HPLC was utilized to quantitate the alkaloids in permeating liquid. Results: In view of the permeation cumulation quantity, the permeation velocity and the lag time of the four kinds of alkaloids, the effect of permeation of purifying extraction was better than extraction with water, and the purifying extraction gel surpassed both the extraction with water and the purifying extraction. Conclusion: It was certified that the purifying extraction gel had improved the effect of permeation of alkaloids, and it is the befitting dosage form for Kushen recipeto treat anaphylaxi disease in skin.