Alkaloid profile and antimicrobial activity of Lupinus angustijolius L.alkaloid extract

摘要

Purpose of the present study was to evaluate alkaloid profile of the aerial parts of Lupinus angustijolius growing in Turkey by capillary gas chromatography-mass spectrometry (GC-MS).Fifteen alkaloids were identified by capillary GC-MS.13alpha-Hydroxylupanine (50.78%) and lupanine (23.55%) were determined as the main alkaloids in the aerial parts of L.angustijolius.Ammodendrine,isoangustifoline,tetra-hydrorhombifoline,angustifoline,alpha-isolupanine,5,6-dehydrolupanine,11,12-dehydrolupanine,13alpha-acetoxylupanine,13alpha-isovaleroyloxylupanine,13alpha-valeroyloxylupanine,13alpha-tigloyloxylupanine,13alpha-cis-cinnamoyloxylupanine and 13alpha-cis-cinna-moyloxy-17-oxolupanine were identified as the minor alkaloids of the plant.Furthermore,antibacterial and antifungal activities of L.angustijolius alkaloid extract were tested against standard strains of the following bacteria;Escherichia coli,Pseudomonas aeruginosa,Bacillus subtilis and Staphylococcus aureus as well as the fungi;Candida albicans and C.krusei.The alkaloid extract showed significant activity on B.subtilis,S.aureus and P.aeruginosa while it was weakly active on E.coli.On the other hand,the extract possessed moderate activity against C.albicans and C.krusei.