Biodegradation Of Endosulfan In Bench Scale Soil Reactors By A Mixed Bacterial Culture

摘要

In the present study, endosulfan-degradation potential of a mixed bacterial culture in soil was examined via bench scale soil reactors. The mixed bacterial culture was enriched from an endosulfan processing industrial surface soil. The soils used for the biodegradation experiments were identified as red soil (GM-Silty gravel with sand) and composted soil (PT-Peat)as per ASTM (American society for testing and materials) standards. Soil applied endosulfan degradation experiments were conducted at an initial endosulfan concentration of 1 mg/g of soil and with an inoculum size of 75 mg/g of soil (OD_(550)). After 28 days, endosulfan degradation efficiency of 92.13 % and 89.16 % was observed in the top zone of red soil and composted soil reactors respectively, where as in the bottom zone, endosulfan degradation efficiency was increased to 95.48 % and 96.03 %. The quantity of endosulfan leached from the red soil reactor (0.185%) was more compared to composted soil reactor (0.095%). The degradation of alpha endosulfan was faster compared to beta endosulfan in all the cases and throughout the study; no known intermediate metabolites were detected in any of the system.