Enzymes are proteins that function as biocatalyst in living systems. One of the major concerns in environmental, biomedical and other applications of enzyme is their short life time restricting its use outside the cell. An effective way to increase the stability and longevity of enzymes from hours to days is to combine them with nanoparticles. With this aim, two different catabolic enzymes-trypsin and peroxidase were attached to the uniform magnetic iron nanoparticles. Uniform magnetic iron nanoparticles were produced from novel cluster deposition technique [1-3]. The sizes of the nanoparticles deposited on petri dish were ～20nm in diameter. The magnetic nanoparticles (MNP) were then attached to the enzymes separately and horseradish peroxide activity (HRP) was measured. The results were compared with the commercially available MNP of size diameter ～100nm. Our study indicates that attached HRP activity of enzymeatic attached to MNP prepared in our lab was compariable and economical in comparison to commercially available nanoparticles. Figure 1 shows that the magnetic nanoparticles - peroxidase complex remained highly stable for three weeks with its enzyme activity in the narrow range of approx. 0.40-0.43U/mg MNPs. Table 1 shows the HRP activity of the enzymes attached to uniform magnetic nanoparticle produced in our laboratory is higher as compared to commercially available magnetic nanoparticles-^sHRP complex.
展开▼
