Human skin UV-induced fluorescence spectra correction on the internal absorption effect

摘要

The reconstruction method of the human skin intrinsic fluorescence spectra distorted by "the screen effect" was proposed. The method is based on the fact that fluorescence and reflectance spectra are formed by the same absorption and scattering properties of the media. Therefore diffuse reflectance spectrum can be used for fluorescence spectrum reconstruction. The intrinsic fluorescence spectral profiles restoration was tested experimentally using scattering media (phantoms, solutions) with a fluorescent dye and hemoglobin as a strong absorber producing inner-filter distortions. The correction produces the fluorescence spectrum profiles same to the one measured from the corresponding sample with an infinitely low absorption. In this paper we present the results of the fluorescence spectra reconstruction using coefficient (R_d(λ))~(1/2) as a correction factor.