Catapulting of microdissected histologic specimens with focused and defocused laser pulses

摘要

We demonstrate by means of time-resolved photography that laser-dissected specimens can be separated from histologic sections by catapulting with focused as well as with defocused laser pulses, and analyze the respective working mechanisms. Both tight focusing and strong defocusing produces minimum damage in the specimen; whereas weak defocusing is less advantageous.