Advances in Phase Detection Technique of Surface Plasmon Resonance Biosensor

摘要

Surface plasmon resonance (SPR) sensing is an optical method based on evanescent wave. SPR biosensor can detect label-free biomolecular interaction in real-time. With further development, it can become a research instrument in proteomics. Phase detection is its most sensitive detection way at present. This paper attempts to summarize the SPR phase detection theory, discuss the major developments, compare the merits and deficiencies of various methods, and look forward to future prospects.