the Detection of SARS-CoV with SPR Biosensor

摘要

SPR (Surface Plasmon Resonance) is an emerging optical biosensor which can monitor the processes at metal interface in real time without labeling requirements. Instrument SPR-2004 can sense two areas in one channel and get two signals at same time in one test. One signal is the graph of the reaction; the other is the graph of reference. SPR biochip is modified with dextran, and only reaction area can be used to immobilize the protein. Two methods of antibody immobilization on chip are tested. One is to immobilize directly, the other is to immobilize protein A firstly and then the antibody is caught by immobilized protein A. The latter is chosen as suitable for retention of the native binding ability with virus of antibody. The signal of detection has reached to 60 units within 40 min. It is 55 units even after reference.