ELISA and Liquid Chromatography/Mass Spectrometry/Mass Spectrometry Methods for Sulfentrazone and Its Acid Metabolite in Groundwater Samples

摘要

Sulfentrazone represents a new class of herbicides (aryl triazolinones). It inhibits the protoporphyrinogen oxidase (PPO) in the plant chlorophyll biosynthetic pathway. The major metabolite identified in the water is sulfentrazone-3-carboxylic acid (SCA). An ELISA (enzyme-linked immunosorbent assay) test kit has been used as an analytical screening tool for the water samples (including well, lysimeter, and other source water) from three groundwater studies, Compared to the conventional chemical assay, ELISA uses minimum chemicals (solvents and reagents) and is simpler and time- and cost-effective. LC/MS/MS (liquid chromatograph equipped with triple quadruple mass spectrometry) was used for the confirmation of all positive and partial negative residues. Method development and comparisons of precision, accuracy, cross-reactivity, recovery, and false negatives and positives for ELISA and LC/MS/MS are discussed herein.