Control techniques of kinesin-driven beads in microfluidic devices

摘要

We have demonstrated manipulation techniques of kinesin-coated beads by integrating orientated microtubules with microfluidic devices. The unidirectional motion of beads was realized with on/off controls on isopolarly oriented and immobilized microtubules. We also succeeded to capture TRITC (tetramethylrhodamine isothiocyanate)-albumin molecules on beads at the intersection of crossed channels and to transport them into the main channel against the buffer flow. Those results provide essential bases for the hybrid nanotransport system powered by the kinesin-microtubule system.