Minimizing the Surface Effect on PCR in PDMS-Glass Chips by Dynamic Passivation

摘要

Dynamic passivation of surfaces of PDMS-glass PCR chips using PEG or PVP was achieved in a conventional thermocycler. The effects of molecular weight of the polymers and the polymer concentration in PCR mixture on surface passivation were investigated. Efficiency of the dynamic passivation follows the order: PVP10000 > PVP55000, PEG8000> PEG10000> PEG400. 0.4% and 0.025% (w/v) are the suitable final polymer concentrations in PCR mixture. The competitive adsorption is the principal activity while the effect of polymers on Taq polymerase is a minor function during the PCR after passivation.