Protoplast cultures of Cucumis and Cucurbita spp.

摘要

The aim of this paper is to present a critical analysis of the available information about protoplasl cultures of Cucurbitaceae with the main focus on Cucumis and Cucurbita spp. Research on protoplast cultures in Cucurbitaceae began in 1975. Since that time, the regeneration of intact plants has been achieved in Cucumis sativus and Cucumis melo by using various isolation and culture protocols. Protoplast fusion led to the development of somatic hybrids of Cucumis melo with Cucurbita moschata x C. maxima, and of Cucumis melo with C. myriocarpus, however these hybrids were sterile. Future trends and progress in Cucurbitaceae protoplast culture research are discussed.