Somatic embryogenesis in a model cultivar, PI 161375 (Cucumis melo subsp. agrestis), of melon

摘要

We investigated a plant regeneration using somatic embryogenesis in two melon cultivars: PI 161375 (Cucumis melo L. subsp. agrestis, chinensis group) and Vedrantais (C. melo L. subsp. melo, ciuitalupensis group). These model cultivars are used for genetic and development studies of melon. As explants, 1- to 4-mm~2 sections of mature seeds were used, including the cotyledon and hypocotyl. To induce embryo formation, the explants were cultured in embryo-induction (EI) medium, which consisted of liquidMS medium containing 2 mg/l 2,4-D and 0.1 mg/l BA. After 4 weeks of culture, an average of 2.7 and 292.3 embryos per seed were produced for Vedrantais and PI 161375, respectively. After 8 weeks of culture, an increased number of somatic embryos were produced for PI 161375. Somatic embryos of Vedrantais were germinated in a higher frequency while those of PI 161375 were in a low frequency.