Biofilms form on materials as a result of bacterial activity.Depending on the situation,these biofilms can have a variety of negative effects on these materials.Therefore,biofilms need to be controlled by the use of appropriate industrial methods.The purpose of this study is to suppress the formation of biofilms by applying weak alternating electromagnetic fields.The project and its results are presented and discussed.For this work,E.coli(a representative gram-negative bacteria present in our daily lives) was selected as the bacteria for forming biofilm.In each case,six samples were prepared.Electromagnetic waves were applied to three of them,while the other three samples did not receive electromagnetic waves.All samples were immersed in a liquid culture (LB culture) at 25 degrees for 5 days.Frequencies used in these experiments were 4 kHz,8 kHz,12 kHz and 16 kHz.The biofilm formation behavior was analyzed by staining with crystal violet and Raman spectroscopy.It seemed to depend on the bacteria and the frequencies used.The results are discussed from the viewpoint of the effect of electromagnetic fields.
展开▼
