Measurement of singlet oxygen luminescence from AML5 cells sensitized with ALA-induced PpIX in suspension during photodynamic therapy and correlation with cell viability after treatment

摘要

Photodynamic therapy (PDT) treatments were performed on suspensions of AML5 cells incubated with ALA-induced PpIX with either 15 mW/cm~2, 45 mW/cm~2 or 90 mW/cm~2 pulsed 523 nm light. Singlet oxygen (~1O_2) luminescence measurements were carried out using a detection system including a near-infrared (NIR) sensitive photomultiplier tube (PMT). Cell viability was determined at several time points during treatments using the propidium iodide (PI) flow cytometry assay. Cumulative ~1O_2 measurements were compared to cell viability for all experiments. Preliminary results show that average cell viability increased with increasing treatment irradiance. Cell viability was found to correlate well with absolute cumulative ~1O_2 luminescence measurements during treatment.