Optical manipulation of microscopic particles and biological cells by laser micro-beams

摘要

The paper introduces to construct a laser micro-beam system for cell manipulation. This system consists of an optical tweezers with optical kinetic effect, and an optical scissor, which is a Nd:YAG laser beam with synthetical effect of sound pressure, thermal expansion and bounce-back pressure. The system can manipulate, deflect, rotate, and punch cells, and so on after we couple two laser beams into microscope. We construct a laser micro-beam system and gained anticipated experiment results. We can successfully manipulate cells in suspension, concise chromosome and punch a hole in cells or organelles on the basis of the system.