Engineering a Mass-spectrometric method for the Analysis of Gene Expression

摘要

Sequence-specific quantification of nucleic acids, primarily for the analysis of messenger RNA (mRNA) transcripts in gene expression studies, is central to functional genomics. As computational methods for making biological inferences from gene expression data become more sophisticated, the demand for high-quality measurements of low-abundance transcripts originating from tightly-regulated genes increases. We have developed a new method for quantifying gene expression denoted as the Mass-spectrometric Analysis of Gene Expression (MAGE).