AN INTEGRATED SSR-STS-SRAP-RAPD GENETIC MAP USING RECOMBINANTINBRED LINE POPULATION IN TETRAPLOID COTTON

摘要

An upland cotton germplasm, NM24016 derived from an interspecific cross between Gossypium hirsutum and G. barbadensewas used to cross with Pima 3-79 to develop a recombinant inbred line (RIL) population. A total of 166 markers, consistingof SSRs, RAPDs, STSs, and SRAPs were developed for the mapping population. Twenty-eight linkage groups were constructedfrom 142 markers. Comparative marker mapping between F2 and RIL populations indicated a linkage map expansionin the RIL population, which strongly supports the strategy to develop the RIL population for more accurate geneticmapping. Other 24 markers that are polymorphic between the two parents did not segregate in the RIL population, indicatingselective elimination on the related chromosomal regions.