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PRESENCE OF NITRIC OXIDE SYNTHASE ACTIVITY UNDER NaCl-INDUCED STRESS IN COTTON CALLUS

机译:NaCl诱导的棉花愈伤液下存在一氧化氮合酶活性的存在

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The presence of nitric oxide synthase (NOS) in cotton callus Coker 312 was studied. The immunoblot analysis of cytosolic extracts with a polyclonal antibody against the C-terminus region of murine iNOS revealed an immunoreactive protein of 130kDa. The evidence presented by measuring the conversion of L-[~(14)C]arginine to L-[14C]citrulline assay indicates the production of nitric oxide as an adaptive mechanism of plants under NaCl induced physiological stress.
机译:研究了棉花蛋糕312中一氧化氮合酶(NoS)的存在。胞质抗体对鼠米莫斯的C-末端区域的细胞溶质提取物的免疫斑分析显示出130kda的免疫反应蛋白。通过测量L-[〜(14)C]精氨酸转化为L-[14C]瓜氨酸测定提出的证据表明,在NaCl诱导的生理应激下生产一氧化氮作为植物的自适应机制。

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