MONITORING INSECT RESISTANCE IN ARKANSAS TO CHEMICALINSECTICIDES AND BT-ENDOTOXINS

摘要

A resistance-monitoring program was established at the University of Arkansas in 2002 to annually gauge changes in majorpest susceptibilities to insecticidal proteins in transgenic crops and shifts in regional and temporal susceptibility to majormodes of action common to critical conventional insecticides. Nineteen colonies of Lepidoptera were collected and assayedfor susceptibility to Cry1Ac and Cry2Ab proteins. The bollworm, Helicoverpa zea, exhibited more variability in susceptibilitythen other species tested. Variation among field colonies was 15-to 22-fold. Laboratory susceptible colonies were 5-to 80-foldmore susceptible then some field colonies. Susceptibilities of field collected insects to acephate, cypermethrin, malathion, andspinosad were measured by a treated glass vial technique. Base-line data were collected for several species of noctuid mothsand important hemipteran pests. An overview of first year results and benchmark data are presented in this paper.