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Improvement of BYDV-PAV Detection in Single Aphid by Using Real-Time RT-PGR Fluorescent Method

机译:用实时RT-PGR荧光法通过改进单蚜虫的BYDV-PAV检测

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Development of biological, serological and molecular tools is a required step for characterization and studying plant viruses. Barley yellow dwarf (BYD), one of the most important disease among small grain cereals (D'Arcy and Burnett, 1995), was observed for the first time in the early 50' based on physiological disturbances of barley plants (Oswald and Houston, 1951). Characterization of the viral agent, Barley yellow dwarf virus (BYDV), involved in this disease was performed using biological, biochemical and biophysical techniques (Rochow and Brakke, 1964; see Smith and Barker, 1999), aphid transmission experiments (Rochow, 1970), serological assays (for a review, see French, 1995) and molecular analysis of the BYDV genome (Miller et al, 1988; Bencharki et al, 1999; Chaloub et al, 1994).
机译:生物,血清学和分子工具的发展是表征和研究植物病毒所需的步骤。 大麦黄矮人(比亚迪)是小谷物谷物中最重要的疾病之一(D'Arcy and Burnett,1995),于50年代初期,在基于大麦植物的生理干扰(Oswald和Houston, 1951)。 病毒剂的表征,涉及这种疾病的大麦黄矮病毒(BYDV),使用生物化学和生物物理技术(Rochow和Brakke,1964;参见Smith和Barker,1999),蚜虫传输实验(Rochow,1970) ,血清学检测(审查,见法文,1995)和BYDV基因组的分子分析(Miller等,1988; Bencharki等,1999; Chaloub等,1994)。

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