PIEZOELECTRIC BIOSENSORS FOR POLYCHLORINATED BIPHENYLS OPERATING IN AQUEOUS AND ORGANIC PHASES

摘要

A novel piezoelectric immunosensor for the determination of polychlorinated biphenyls (PCBs) is described.In the direct assay format adopted,we used sheep anti-PCB antibody immobilized on the surface of optically smooth gold electrodes,which were vacuum deposited on quartz crystal resonators.The sensing surface was first modified with a self-assembled monolayer of dithiobis(succinimidyl thio-propionic acid) allowing for a covalent attach-ment of Protein A.