Lignophenol was prepared from extractive-free wood meal in the two phases system composed of p-cresol and 72% sulfuric acid. During this process, carbohydrates were swollen, followed by partial hydrolysis and dissolution in the acid solution, resulting in the decomposition of interpenetrating polymer network structure in the cell wall. Lignin solvated with cresol contacts with acid for a short time only at the interface between sulfuric acid and cresol, whereby the cleavage of benzyl aryl ethers and selective phenolation at a -positions occur. The results lead native lignin to highly phenolic diphenylmethane type materials, which still retain basic structures of native lignin. In comparison with the native lignin, lignophenol contains more phenolic hydroxy groups, so lignophenol derivatives have high affinity for proteins. Papain and cellulase in water solution could be absorbed by lignophenol. The lignophenol-enzyme complexes were very stable in aqueous systems. The two kinds of enzyme could be easy released by changing an organic solvent. The formation of the lignophenol-enzyme complexes was the result of the super-molecular interaction between lignophenol and the two kinds of enzyme.
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