COMPETITIVE AND SANDWICH TIRF IMMUNOSENSORS: A MODEL FOR PERFORMANCE OPTIMIZATION

摘要

The effects of some fundamental parameters on the performance of optical immunosensors based on fluorescence induced by an evanescent wave (Total Internal Reflection Fluorescence, or TIRF) are evaluated. Both competitive and sandwich systems are discussed and compared. The performance of such devices are described using the range within which the error is less than a fixed value. Methods of controlling the effects of various parameters are considered.