Application of island films technique to fluorescence studies of biological structures

摘要

In this work we studied fluorescence properties of pyrene and pyrene in microsomal membranes, adsorbed on the surface of silver island film (SIF). Pyrene adsorbed on SIF from acetonitrile possesses enhanced emission in the region 420-500 nm. Fluorescence of pyrene monomers (360 - 400 nm) is negligible. Microsomal membranes probed by pyrene were adsorbed on a quartz plate and silver island film surfaces. In the former case the fluorescence intensity of pyrene monomer form is greater. It was found that the effect of fluorescence enhancement significantly depends on spectral properties of rough silver surface. Heat treated (annealed) according to special technique silver island films were the most suitable for fluorescence studies. It is shown that fluorescence enhancement effect is determined by the interaction of pyrene π-electron system with surface plasmons of SIF. On the base of obtained results the possibility of rough metal surfaces application to fluorescence studies of microsomal membranes is discussed.