This paper introduced a basis principle of an autofocus system, including the hardware composition and the software flow chart. The most important work of an autofocus system is to construct or select a suitable focus function. We first discussed some traditional focus functions, pointed out their advantages and shortages. Then we proposed a new autofocus algorithm, which based on wavelet transform. We use the diagonal edge information of the result of wavelet transform to form focus function. After many experiments we draw a conclusion that the wavelet transform method is superiority than others, use it can get a highly accuracy along with a fast speed, special in the biomedical microscopic image analysis. At the last of this paper we discussed the further work related to the improvement of hardware condition and the optimization of focus function.
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