Experimental Research of Photodynamic Diagnosis Mediated by Chemiluminescence Reagents

摘要

There are some serious limitations existing in conventional photodynamic diagnosis (PDD), such as autofluorescence disturbance, weak red-band absorption efficacy of traditional photosensitizers and quite short penetration depth of excitation light. In this paper a novel method of PDD mediated by chemiluminescence reagents is presentedi n fabricated mixed samples. Photodynamic therapy (PDT) is believed to act via singlet oxygen (~1O_2) generated by energy from photosensitizers~(1). 5-Fluoresceinyl Cypridina Luciferin Analog (FCLA), a chemiluminescence reagent, can react with singlet oxygen and emit chemiluminescence~(3) with peak wavelength about at 526 nm~(4). In the experiments, FCLA was chosen as an optical reporter of localization of singlet oxygen produced from photosensitized reaction of Hematoporphyrin Derivative (HpD) excited by 630 nm laser. Experimental results, the measurement of fluorescence spectra and the detection of iamge from chemiluminescnece, show that the mixed HpD & FCLA in biologic tissue mixture excited with the laser of 630 nm can emit detectable light signals.