IN this paper, biological sonoluminescence (SL) of different tissues was investigated for the first time. Tissue samples (muscle, liver, and fat) were prepared and put into a column-shaped ultrasound field. A photomultiplier tube and a back-illuminated cooled CCD were used to detect the SL signal. In the experiments, FCLA (Fluoresceinyl Cypridina Luminescent Analog) was used to enhance SL. With the chemiluminescence technique, we suggest that the biological SL mainly come from the free radicals produced by the ultrasound cavitation, which is different from the mechanism of the SL in aqueous solutions.
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