DNA Detection Method by Analyzing DNA-labeled Microbead Velocity under Traveling Wave Dielectrophoresis

摘要

We have developed a rapid DNA detection method based on the dielectrophoresis (DEP) characteristic of DNA labeled microbeads. The surface conductance of microbeads is affected by the DNA labeling and causing the change of Clausius-Mossotti (CM) factor K of DEP. In former studies，we focused on the effect of the real part of K (Re[K]). Recently，we proposed a new DNA detection method based on the traveling wave dielectrophoresis (twDEP)， which is a phenomenon mainly effected by the imaginary part of K (Im[K])，because the Im[K] will change more dramatic than Re[K] against DNA attachment on the microbeads. In this study， the velocity of DNA labeled microbeads under twDEP force was measured by computer-based image analysis.