At the optical processing domain, the three-dimension reconstruction of information is an important problem of research. At the last few years, the laser confocal scanning microscope (LCSM) system was researched and has been used successfully as advanced optical instrument in the biological and medical research range. This paper primarily research the 3-D image reconstruction of the cell using its 2-D section image formed by the laser confocal scanning microscopy. First, the paper rests on the LCSM system characteristic of forming image to analyze the image noise and remove it. Then we extract the information of 2-D section image edge, and we use these informations to reconstruct the 3-D image of cell surface by method of B-Spline.
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