A New Chemo Enzymatic Synthesis: Bioconversion of Tran-P-Arylacrylic Acid to L-β-Aryl-α-AIanine by L-Phenylalanine Ammonia Lyase of Rhodotorula

摘要

A new route to the Chemo Enzymatic asymmetric synthesis of L-β-aryl-α-alanine was developed by using the red yeast (Rhodotorula rubra AS 2.166, Rhodotorula glutinis AS 2.102, Rhodotorula sp. CIBAS A 1401) cells containing L-Phenylalanine ammonia lyase (PAL, EC 4.3.1.5) activity as biocatalysts to catalyze the addition of ammonia to olefinic bond in trans-arylacrylic acid, and prier to twenty four compounds were chemically synthesized, and by means of bioconversion fourteen of L-α-amino acid were produced with the conversion rates from 7.9 to 90.0 percent. In this process, their reactive parameters and kinetics were studied under the optimum reactive conditions selected.