A rapid and quantitative method of quality assurance for the marker phytochemicals and bioactive components in products containing materials derived from Goldenseal has been developed. By using a Luna phenyl-hexyl column with a mixture of sodium lauryl sulfate-phosphoric acid-water-isopropanol-acetonitrile as mobile phase, the two marker compounds, hydrastine and berberine were well separated at ambient temperature in 20 minutes. The alkaloid contents of six commercial available Goldenseal root and Goldenseal/Echinacea supplements were also analyzed.
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