Quality control of instrumentation in videomicroscopy incidence on DNA analysis

摘要

Recent advances in video microscopy in the field of cytopathology need to define in detail requirements for rigorous technical quality control of DNA analysis. This study deals with the influence of both quality and calibration of instrumentation on DNA content measurements. Quality control of instrumentation in stability, linearity, and shading estimations has been achieved for two SAMBA$+TM$/2005 (ALCATEL-TITN-Answare/France) image analysis systems by using prototype reference standard slide and Feulgen-stained rat liver imprints. The accuracy of the various conditions of work has been expressed as the CV of integrated optical density (IOD) and varied from 1.5% to 5.4%. These results demonstrate the dependence of the accuracy of measurements on the conditions for calibration of the instrument. Moreover, this work shows that each system can be characterized by its specific answer and all the systems don't really offer acceptable conditions for nuclear DNA content determination.