Until recently, the ability to measure the changing oxygen gradients in perfused tissues in response to metabolic demand, has been limited to point-measurements and/or averaged A-V oxygen differences during perfusion using oxygen electrodes. With the recent introduction of novel phosphorescent probes specifically quenched by oxygen, the ability to spacially map oxygen gradients in real-time may offer new insights into the dynamics of microvascular design and supply. Accordingly, this paper provides initial image data on Langendorff perfused rat hearts wherein the relative change in phosphorescent intensity of Pd-meso-tetra(4- carboxyphenyl)phorphine (2$mu@m) as the reporter probe, is quantitatively related to spacial oxygen gradients as seen on the left-ventricle during changing gassing conditions. Digital image analysis (frame advance), after proper calibration and alignment, provides images which can be usefully interpreted. Clinical applications of such emerging technologies could have wide-spread diagnostic applications not only as applied to the coronary bed, but other tissue surfaces displaying various degrees of aschemia and/or hypoxia.
展开▼
机译:直到最近,响应于代谢需求测量灌注组织中变化的氧梯度的能力已经限于使用氧电极在灌注期间的点测量和/或平均A-V氧差异。随着最近引入新的磷光探针,特别是通过氧明细淬火,可以实时地将氧气梯度定位的能力可以为微血管设计和供应的动态提供新的见解。因此,本文提供了Langendorff灌注大鼠心脏的初始图像数据,其中Pd-Meso-Tetra(4-羧基)卟啉(2 $ MU @ M)作为报告探针的磷光强度的相对变化是与空间氧的定量相关在更换气体条件期间,左心室看到的梯度。数字图像分析(帧前进)在适当的校准和对准后,提供可以有用地解释的图像。这种新兴技术的临床应用可能具有广泛的诊断应用,不仅适用于冠状动脉床,而且具有呈现各种疏水症和/或缺氧的其他组织表面。
展开▼