The distortion analysis and correction of two-dimensional scanning system in laser differential confocal microscopy

摘要

Laser differential confocal microscopy (DCM) is widely applied to many fields as an important analysis and measurement approach for its high-resolution imaging capability and unique section imaging capability. Differential confocal microscopy needs a scanning system to achieve the imaging due to its zero-field imaging property. So two-dimension vibrating mirror scanning system with fast-speed positioning and high response frequency is wildly used for fast speed scanning system. . However, the two-dimension vibrating mirror scanning system may bring the distortion to the image. Therefore, a mathematic model of the distortion for two-dimension vibrating mirror scanning system is built in this paper. And an inverse function method for correction is put forward and the corresponding theory simulation has been done using MatLab. The proposed method can be operated easily and understood easily, and can be applied to large-scale calculation correction, which can provide an effective way to correct the distortion of two-dimension vibrating mirror scanning system.